Koret School of Veterinary Medicine
Robert H. Smith Faculty of Agriculture, Food and Environment,
The Hebrew University of Jerusalem
Daniel Barkan, M.D.
Mycobacterial pathogenesis research
Human and animal mycobacterial diseases, including human and bovine tuberculosis, leprosy, Johne's disease and others.
2000: M.D, Hadassah Medical School, Jerusalem.
Mycobacterium tuberculosis is a major human pathogen, causing the widespread disease of tuberculosis. It latently infects 1/3 of the world's population, and causes between 1 and 1.5 million deaths per year worldwide. Mycobacterium bovis is a close relative of M.tb, sometimes causing disease in humans but mostly infecting livestock, especially cattle. It is a major animal pathogen, with a huge economic burden.
In our lab we study virulence factors of both M. tb and M. bovis. We try to discover genes and pathways important for their ability to cause disease, and interrupt these genes using molecular techniques to produce non-pathogenic mutants, with potential to serve as vaccines against these diseases.
We cooperate with laboratories in the USA, Belgium and Russia in our studies.
Barkan D, Liu Z, Sacchettinni JC, Glickman MS. (2009) Mycolic Acid Cyclopropanation is Essential for Viability, Drug Resistance, and Cell Wall Integrity of Mycobacterium tuberculosis. Chemistry & Biology 16, 499–509
Barkan D, Rao V, Sukenick GD, Glickman MS. (2010). Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis cis-cyclopropanation of oxygenated mycolates. Journal of Bacteriology. July; 3661–3668.
Barkan D, Stallings CS, Glickman MS: (2010) An improved counterselectable marker system for mycobacterial recombination using galK and 2-Deoxy-Galactose. Gene; (470) 31-6.
Gupta R, Barkan D, Redelman-Sidi G, Shuman S, Glickman MS: (2011) Mycobacteria exploit three genetically distinct DNA double-strand brake repair Pathways. Molecular Microbiology, Jan; 79(2) :316-30.
Barkan D, Hedhli D, Han-Guang Y, Huygen K, Glickman MS: (2012) M. tuberculosis lacking all mycolic acid cyclopropanation is viable but highly attenuated and hyperinflammatory in mice. Infection and Immunity, Jun;80(6):1958-68